tissuescan prostate cancer tissue array ii  (OriGene)

Journal: Heliyon

Article Title: Novel roles for HMGA2 isoforms in regulating oxidative stress and sensitizing to RSL3-Induced ferroptosis in prostate cancer cells

doi: 10.1016/j.heliyon.2023.e14810

Figure Lengend Snippet: HMGA2-WT and -TR Expression in PCa and Breast cancer Cell Lines and Patients Tissue (A) Western blot analysis shows the expression of HMGA2 protein in prostate cancer and breast cancer cell lines, HMGA2-WT displays a higher molecular weight at about 23 KDa while the HMGA2-TR displays a lower molecular of about 17 KDa. (B) Western blot analysis measured the protein levels of both HMGA2-WT and -TR in prostate cancer patient tissue displaying different Stage/Gleason scores. (C) RT-PCR measured the mRNA levels in cDNA array. (D) Immunohistochemical analysis was performed using prostate tissue microarray with HMGA2 antibody in normal prostate tissue, hyperplasia, and chronic inflammation prostate tissue. Scale bar; 100 μm. Magnification 20X, inset 40X. Statistical analysis was conducted utilizing GraphPad Prism; (**** p < 0.0001, ***p < 0.001, **p < 0.01). Bars represents SD of the mean. Alpha-tubulin was used as a loading control. Results are representative of 3 independent experiments.

Article Snippet: Prostate normal and cancer tissue cDNA array (TissueScan, Prostate Cancer cDNA Array III- CAT#: HPRT103) was purchased from OriGene (Rockville, MD).

Techniques: Expressing, Western Blot, Molecular Weight, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining, Microarray, Control